Webantibody (Bioworld, AP0064). Monoclonal antibody against human FasL, NOK-2, was purchased from BD Pharmingen (San Diego, CA, USA). Dabco 4, 6-diamidino-2-phenylindole (DAPI) was purchased from Beyotime (Shanghai, China). In situ cell death detection kit (Roche Applied Science, Mannheim, Germany, #11684795910) was … WebAntibodies used in Western blotting: CD 1 lb antibody (Novus biologicals, NB 110-89474), α-Tubulin antibody (Bioworld, AP0064). Methods a) Cell culture: BV2 cells were grown in DMEM containing 10% fetal bovine serum (FBS), placed in 5% CO 2 , cultured in a 37 °C constant temperature incubator (relative humidity was 95%), and observed under ...
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