Facs-based crispr/cas9 screen
WebJan 1, 2016 · Here, we describe a genome-wide CRISPR/Cas9 screen in immortalized mouse macrophages aiming at the unbiased identification of gene products involved in Nlrp3 inflammasome activation. We employed a FACS-based screen for Nlrp3-dependent cell death, using the ionophoric compound nigericin as a potassium efflux-inducing stimulus. WebApr 12, 2024 · Using a fluorescence-activated cell sorting (FACS)-based CRISPR/Cas9 screen, we identified HIF-1α as a substrate of the cleavage and polyadenylation specificity factor-1 (CPSF1), an E3-ligase which targets HIF-1α for degradation in the presence of an ABL kinase inhibitor in Hx...
Facs-based crispr/cas9 screen
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WebNov 6, 2024 · CRISPR screening is a large-scale genetic loss-of-function experimental approach designed to find the equivalent of a few needles in a haystack. CRISPR screening facilitates discovery of key genes or … WebCas9 screen技术是一项极具潜力的高通量筛选工具，已被应用于各个领域。 同时，其衍生与拓展技术，如Cas13 screen、Base editor screen也逐步问世。 我相信未来的研究手 …
WebNov 22, 2024 · Unbiased genome-wide loss-of-function screens are powerful approaches to identify new regulators of physiological processes. In particular, CRISPR-Cas9–based genome-wide pooled screening techniques have democratized this strategy for use in mammalian cells because of their excellent genomic coverage, relatively low cost, and … Web• Developed an assay by using CRISPR/Cas9 mediated gene-disruption combined with high-throughput flow cytometry-based competition …
WebThe MAGeCK (Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout) algorithm was published in 2014 and is one of the most popular comprehensive methods for … Web• Developed an assay by using CRISPR/Cas9 mediated gene-disruption combined with high-throughput flow cytometry-based competition …
WebJan 25, 2024 · A high-throughput FACS-based screen was developed to identify host proteins required for C. trachomatis invasion (i.e., attachment and entry) into human cells …
WebJan 22, 2024 · Phagocytic clearance of dying cells, termed efferocytosis, must occur efficiently to maintain homeostasis and prevent disease. Yet, our understanding of this important biological process remains incomplete. To search for novel regulators of efferocytosis, we performed a FACS-based genome-wide CRISPR knockout screen in … psx memory toolWebJul 25, 2024 · FACS-based genome-wide CRISPR/Cas9 screen for bacterial autophagy is presented in Figure 3D. The candidate genes were determined based on the criteria that at least two or more gRNAs are enriched with the maximum fold change (FC) among three separate experiments being above 40. The gRNA hits are ranked by the median FC from … horticultural beansWebEarly attempts to apply CRISPR-Cas9 for genome editing in human primary T cells used either viral vectors 1,2 or plasmids 3,4 for Cas9 and gRNA expression, resulting in low targeting efficiency and high toxicity. More recently, ribonucleoprotein (RNP)-based CRISPR-Cas9 expression systems have achieved high efficacy across a number of … horticultural bone mealWebApr 10, 2024 · Here, using a genome-wide FACS-based CRISPR-Cas9 screen, we identify almost all known positive regulators of mTORC1 as well as many others. The results of our screens highlighted the importance of ... psx keeps crashingWebNov 14, 2024 · We call this technique FACS-assisted CRISPR-Cas9 editing (FACE). FACE allows the derivation of correctly edited polyclones carrying a positive selection fluorescent module and the exclusion of non-edited, random integrations and on-target allele NHEJ-containing cells. We derived a set of isogenic lines containing Parkinson's-disease … psx mem toolWebJan 19, 2024 · We have combined this optical enrichment strategy with pooled CRISPR-Cas9 libraries to perform imaging-based CRISPR screens. ... For FSC and H2B-mGFP screens, cells were then subjected to FACS sorting. Cells before FACS (unsorted sample for FSC and H2B-mGFP screens) and top 10% cells based on either FSC signal (high … psx marketwatchWebHere, we perform a genome-wide fluorescence-activated cell sorting (FACS)-based CRISPR-Cas9 screen in human cells and identify genes encoding components of the lysosomal vacuolar ATPase (v-ATPase ... psx merchant finder